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KMID : 0359319750150010051
Korean Journal of Veterinary Research
1975 Volume.15 No. 1 p.51 ~ p.55
Studies on Resistance of Embryonated Eggs , First - Stage Larvae , and Free - Living Infective Larvae of Metastrongyus apri to Different Temperatures

Abstract
In this study two sets of experiments were undertaken. Firstly, the embryonated eggs and first-stage larvae, sprayed in disinfected soil, were exposed to different temperatures. Then, the viabilities of them were weekly determined by inoculating the soil to earthworms, Eisenia foetida. Secondly, the infective larvae, seperated from the earthworms and suspended in tap water, were also exposed to different temperatures and their viabilities were checked microscopically at weekly intervals.
The results were summerized as follows:
1. The maximum longevities of embryonated eggs and first-stage larvae were determined as 1 week at 35¡É, over 36 weeks at 25¡É, 15¡É, and 5¡É,32 weeks at -5¡É, and under 1 week at -15¡É.
2. The mean-numbers of infective larvae detected from the test earthworms were greatest at 5¡É, and decreased with rise or fall of the temperature.
3. Infective larvae freed from the intermediate host were able to survive for 2 weeks at 25¡É and 3 weeks at 15¡É. However, they lost their viabilities in a week at 35¡É, 5¡É, -5¡É, and -15¡É.
4. The number of living infective larvae at 15¡É was greater than at 25¡É.
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